INTRACELLULAR DISTRIBUTION OF TRICARBOXYLIC ACID CYCLE ENZYMES IN AZOTOBACTER VINELANDII

Intracellular distribution of tricarboxylic acid cycle enzymes in Azotobacter vinelandii.

The cytological complexity of the bacterial cell is well established, but little information is as yet available on the association of metabolic function with cellular structure in microorganisms. The significance of the tricarboxylic acid (TCA) cycle and the electron transport system in the metabolism of the obligate aerobic azotobacter cell makes important a knowledge of the intracellular dis...

Distribution of tricarboxylic acid cycle enzymes and glyoxylate cycle enzymes between mitochondria and peroxisomes in Tetrahymena pyriformis.

Tetrahymena Pyriformis cells were grown axenically, harvested in the stationary phase of the cultures when the organism is glyconeogenetically active, homogenized, and fractionated either by isopycnic density gradient centrifugation in a sucrose gradient or by zonal differential sedimentation through a sucrose gradient. Protein and enzyme determinations carried out on the fractions led to the f...

Tricarboxylic acid cycle enzymes of the ectomycorrhizal basidiomycete, Suillus bovinus.

In crude cell extracts of the ectomycorrhizal fungus, Suillus bovinus, activities of citrate synthase, aconitase, isocitrate dehydrogenase, succinate dehydrogenase, fumarase, and malate dehydrogenase have been proved and analyzed. Citrate synthase exhibited high affinities for both its substrates: oxaloacetate (Km = 0.018 mM) and acetyl-CoA (Km = 0.014 mM). Aconitase showed better affinity for ...

Tricarboxylic Acid Cycle in

Genes in Azotobacter vinelandii

Azotobacter vinelandii contains a heterodimeric, membrane-bound [NiFelhydrogenase capable of catalyzing the reversible oxidation of H2. The 13 and at subunits of the enzyme are encoded by the structural genes hoxK and hoxG, respectively, which appear to form part of an operon that contains at least one further potential gene (open reading frame 3 1ORF3]). In this study, determination of the nuc...